Her first appearance is in Houseof Slendrina. She also appears, and becomes a threat, in Slendrina: The Asylum. From this game onwards she becomes a constant threat. She has also appeared in Slendrina 2D, Slendrina: The Forest, Slendrina: The Cellar 2, and Slendrina X. In Slendrina: The Asylum, she, besides Slendrina, is the only antagonist in the game. She mainly wanders the asylum until she finds the player or when the player moves too close to her. When she spots the player, she will chase the player in attempting to slap him. In Slendrina 2D, she attempts to strike the player when he manages to get to the room that contains a metal rod. The only way to get passed her is to use the metal rod to defend. In Slendrina: The Cellar 2, she, along with Slendrina's child, wander around the abandoned cellar until they find the player, in which they try to not let them escape by attacking them. In Slendrina X, she is seen chained up inside of a cage, unmoving. When the player leaves the room, she breaks free, but she does not become hostile until the escape sequence. In House of Slendrina, she is seen, and whenever you enter either of the bedrooms in Slendrina's house, she will scream. If you go in her closet she will lock you in for a few seconds but she will eventually let you out and when you walk out she will be sitting on the bed, hunched over and convulsing as though she is about to vomit. If you get closer Slendrina will jump scare you by staring at the player, covering the camera entirely, but this does not harm you.
In Granny, Slendrina's mom appears as an Easter egg, similar to Slendrina's appearance in Granny.
To reveal this Easter egg, the player has to knock the painting in bedroom 2 off of the wall, revealing the bookshelf lever. When this lever is pulled, the bookshelf will move, giving the player access to the bookshelf room. Inside this room is a pedestal. If the book is placed on the pedestal, the back wall will shift backwards, revealing Slendrina's mom's body, who is chained to the wall, similar to her appearance in Slendrina X. She cannot harm the player. She also appears in a painting in the crow's room.
- Just like the Slendrina Easter egg, if Granny is awake, she will disappear and reappear in the basement.
- The painting of Slendrina's mom came from the ending of Slendrina: Asylum, although the one in Granny is in landscape instead of portrait.
- Moreover it is not known what the relationship was like between the two, as Granny appears to have an adversarial relationship with Slendrina's mom, having her chained to a wall in a hidden room.
- Though this theory may be false, as Granny has a painting of her hanging up in the crow's room.